NUDC antibody (AA 176-267)

Catalog No. ABIN7600299

This Rabbit Polyclonal antibody specifically detects NUDC in WB, ELISA, IF, ICC and FACS. It exhibits reactivity toward Human.

Quick Overview for NUDC antibody (AA 176-267) (ABIN7600299)

Target: NUDC

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUDC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-NUDC Antibody

Binding Specificity: AA 176-267

Purpose: Anti-NUDC Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NUDC Antibody Picoband® (ABIN7600299). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NUDC recombinant protein (Position: Q176-K267).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Kikuno, R., Nagase, T., Ishikawa, K., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XIV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 6: 197-205, 1999. 2. Zhou, T., Zimmerman, W., Liu, X., Erikson, R. L. A mammalian NudC-like protein essential for dynein stability and cell viability. Proc. Nat. Acad. Sci. 103: 9039-9044, 2006.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NUDC

Target: NUDC

Background:

Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-1, AMPK subunit alpha-1

Tissue Specificity: Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

Background: Nuclear migration protein nudC is a protein that in humans is encoded by the NUDC gene. This gene encodes a nuclear distribution protein that plays an essential role in mitosis and cytokinesis. The encoded protein is involved in spindle formation during mitosis and in microtubule organization during cytokinesis. Pseudogenes of this gene are found on chromosome 2.

Molecular Weight: 43 kDa

Gene ID: 10726

UniProt: Q9Y266